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Rabbit抗原名称
SIGMAR1分子别名
Sigma non-opioid intracellular receptor 1; Aging-associated gene 8 protein; SR31747-binding protein (SR-BP); Sigma 1-type opioid receptor (SIG-1R; Sigma1-receptor; Sigma1R; hSigmaR1); OPRS1; SRBP免疫原
Synthetic Peptide细胞定位
Nucleus membrane, Cell membrane, Endoplasmic reticulum membraneAccession
Q99720克隆号
S-2079-270抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, 293T, Caco-2, NIH/3T3, mouse liver, mouse brain, C6, rat liver, rat brain, COS-7预测反应种属
(反应种属缩写表)Bv, GP, Ft纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt, Mk ICC 1:100 Hu, Ms FCM e
The SIGMAR1 protein, also known as the sigma-1 receptor, is a multifunctional chaperone protein composed of 223 amino acids with a molecular mass of 25.3 kDa. It is encoded by the SIGMAR1 gene and is ubiquitously expressed in various tissues, including the brain, heart, liver, and kidneys. SIGMAR1 is primarily located in the mitochondria-associated endoplasmic reticulum membrane (MAM), where it regulates calcium signaling, ion channel activity, and mitochondrial function. This protein plays a crucial role in cellular survival and has been implicated in several diseases, such as neurodegenerative disorders (e.g., amyotrophic lateral sclerosis and Alzheimer's disease), cardiovascular diseases, and certain cancers. Additionally, SIGMAR1 has been shown to have protective effects against neuronal and cardiac injuries.
免疫印迹
WB result of SIGMAR1 Recombinant Rabbit mAb
Primary antibody: SIGMAR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: Caco-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 28 kDaWB result of SIGMAR1 Recombinant Rabbit mAb
Primary antibody: SIGMAR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 28 kDaWB result of SIGMAR1 Recombinant Rabbit mAb
Primary antibody: SIGMAR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat liver lysate 20 µg
Lane 3: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 28 kDaWB result of SIGMAR1 Recombinant Rabbit mAb
Primary antibody: SIGMAR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 28 kDa
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- SIGMAR1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- SIGMAR1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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