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宿主来源
Rabbit抗原名称
Slug分子别名
Zinc finger protein SNAI2; Neural crest transcription factor Slug; Protein snail homolog 2; SLUG; SLUGH; SNAI2细胞定位
NucleusAccession
O43623克隆号
S-3177抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
A204, NIH/3T3纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms
Slug protein is a transcription factor that plays a crucial role in various biological processes. It belongs to the Snail family of zinc-finger transcription factors and is highly conserved across different species. Slug is involved in epithelial-mesenchymal transition (EMT), a process where epithelial cells lose their cell-cell adhesion properties and acquire mesenchymal characteristics This. transition is essential during embryonic development for tissue remodeling and organogenesis. In addition, Slug has been implicated in cancer progression, where it can promote tumor invasion and metastasis by downregulating the expression of cell adhesion molecules such as E-cadherin. It also has roles in maintaining stem cell properties and regulating cell survival under stress conditions.
免疫印迹
WB result of Slug Recombinant Rabbit mAb
Primary antibody: Slug Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Lane 2: A204 whole cell lysate 20 µg
Negative control: PANC-1 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 35 kDaWB result of Slug Recombinant Rabbit mAb
Primary antibody: Slug Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 35 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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