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Rabbit抗原名称
XBP-1s分子别名
X-box-binding protein 1; XBP-1; Tax-responsive element-binding protein 5 (TREB-5); TREB5; XBP2; XBP1免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P17861克隆号
S-2341-53抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa treated with Tunicamycin, mouse liver, rat liver纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt
XBP-1s protein is a key transcription factor involved in the unfolded protein response (UPR), a cellular stress response pathway activated when the endoplasmic reticulum (ER) is overwhelmed with misfolded proteins. It is generated from the precursor XBP-1 mRNA through a unique splicing event triggered by ER stress, resulting in the production of the active XBP-1s isoform. XBP-1s plays a crucial role in promoting the expression of genes involved in ER protein folding, degradation of misfolded proteins, and lipid biosynthesis, thereby helping to restore ER homeostasis. Dysregulation of XBP-1s has been implicated in various diseases, including metabolic disorders, neurodegenerative diseases, and cancer, highlighting its importance in maintaining cellular health and function.
免疫印迹
WB result of XBP-1s Recombinant Rabbit mAb
Primary antibody: XBP-1s Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 2 μg/ml Tunicamycin for 8 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 60 kDa
This blot was developed with high sensitivity substrateWB result of XBP-1s Recombinant Rabbit mAb
Primary antibody: XBP-1s Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 65 kDaWB result of XBP-1s Recombinant Rabbit mAb
Primary antibody: XBP-1s Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 65 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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