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Rabbit抗原名称
Mrp4分子别名
ATP-binding cassette sub-family C member 4; MRP/cMOAT-related ABC transporter; Multi-specific organic anion transporter B (MOAT-B); Multidrug resistance-associated protein 4; MOATB; MRP4; ABCC4免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
O15439克隆号
S-2305-17抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms IHC-P 1:1000 Hu
Mrp4 protein, also known as multidrug resistance protein 4, is a member of the ATP - binding cassette (ABC) transporter family. It is an integral membrane protein that plays a crucial role in the efflux of various substances out of cells. Mrp4 can transport a wide range of substrates, including organic anions, nucleoside - derived metabolites, and certain drugs. This protein is expressed in multiple tissues such as the liver, kidneys, and intestines. Its function is important for maintaining cellular homeostasis by preventing the accumulation of potentially toxic substances within cells. Additionally, Mrp4 has implications in the pharmacokinetics of drugs, as it can influence the absorption, distribution, metabolism, and excretion of certain medications. In the context of disease, alterations in Mrp4 expression or function have been associated with drug resistance in cancer cells and may also be involved in the pathogenesis of some renal disorders.
免疫印迹
WB result of Mrp4 Recombinant Rabbit mAb
Primary antibody: Mrp4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled A549 whole cell lysate 20 µg
Lane 2: unboiled LNCaP whole cell lysate 20 µg
Lane 3: unboiled SW620 whole cell lysate 20 µg
Lane 4: unboiled HT-29 whole cell lysate 20 µg
Lane 5: unboiled U-2 OS whole cell lysate 20 µg
Lane 6: unboiled OVCAR-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 150 kDa
Observed MW: 170-280 kDaWB result of Mrp4 Recombinant Rabbit mAb
Primary antibody: Mrp4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled NIH/3T3 whole cell lysate 20 µg
Lane 2: unboiled J774A.1 whole cell lysate 20 µg
Low expression control: unboiled NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 150 kDa
Observed MW: 170-250 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Mrp4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-Mrp4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Mrp4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-Mrp4 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Mrp4 in tumor tissue.
Expression of Mrp4 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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