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Rabbit抗原名称
Perforin分子别名
Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein; Pfp; Prf1免疫原
Recombinant Protein细胞定位
Cell membrane, SecretedAccession
P10820克隆号
S-1944-68抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
CTLL-2, mouse spleen, rat spleen纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Ms, Rt
Perforin is a crucial protein involved in the immune response, primarily produced by cytotoxic T lymphocytes and natural killer cells. It functions by forming pores in the membranes of target cells, such as virus-infected or cancerous cells. Once released from immune cells, perforin molecules aggregate and insert into the target cell membrane, creating channels that disrupt the cell's osmotic balance, leading to cell lysis and death. This mechanism is essential for eliminating harmful cells and maintaining the body's overall health.
免疫印迹
WB result of Perforin Recombinant Rabbit mAb
Primary antibody: Perforin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: CTLL-2 whole cell lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Negative control: C2C12 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 60-80 kDaWB result of Perforin Recombinant Rabbit mAb
Primary antibody: Perforin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 62 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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