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PXR Recombinant Rabbit mAb (S-2398-43)

Nuclear receptor subfamily 1 group I member 2,Orphan nuclear receptor PAR1,Orphan nuclear receptor PXR,Pregnane X receptor,Steroid and xenobiotic receptor (SXR),NR1I2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6148

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

PXR
分子别名

Nuclear receptor subfamily 1 group I member 2; Orphan nuclear receptor PAR1; Orphan nuclear receptor PXR; Pregnane X receptor; Steroid and xenobiotic receptor (SXR); NR1I2
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

O75469
克隆号

S-2398-43
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, HepG2, THP-1, mouse liver, rat liver
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ChIP	1:20-1:50	Hu

背景介绍

PXR protein (Pregnane X Receptor) is a nuclear receptor that plays a crucial role in the regulation of drug metabolism and detoxification processes in the body. It is primarily expressed in tissues such as the liver and intestines. When activated by various endogenous and exogenous compounds, including certain drugs, PXR can induce the expression of numerous target genes. These target genes are involved in the production of enzymes like cytochrome P450, which are key for the biotransformation of drugs and other xenobiotics. This helps the body to effectively metabolize and eliminate potentially harmful substances. PXR's activity is important for maintaining homeostasis and ensuring the proper functioning of the body's detoxification systems.

免疫印迹
- WB result of PXR Recombinant Rabbit mAb
  Primary antibody: PXR Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: THP-1 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 50 kDa
  Observed MW: 50 kDa
- WB result of PXR Recombinant Rabbit mAb
  Primary antibody: PXR Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 50 kDa
  Observed MW: 50 kDa
- WB result of PXR Recombinant Rabbit mAb
  Primary antibody: PXR Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 50 kDa
  Observed MW: 50 kDa
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HepG2 cells cross-linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used PXR Recombinant Rabbit mAb (S-2398-43) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post -immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of HMGN4, HES7 and SAT-α in PXR Recombinant Rabbit
  mAb (S-2398-43)-immunoprecipitated sample.