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Rabbit抗原名称
VSV G分子别名
Glycoprotein G; G免疫原
Recombinant ProteinAccession
P0C2X0抗体类型
Polyclonal antibody抗体同种型
IgG纯化方式
Immunogen Affinity浓度
1 mg/ml纯度
> 95% by SDS-PAGE标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
2 to 8°C for 12 months under sterile conditions;
-80°C for 36 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.应用
ELISA
稀释度
应用 稀释度 推荐种属 ELISA 1:1000-1:5000 VSV
The VSV G protein, which stands for vesicular stomatitis virus glycoprotein, is a crucial component of the vesicular stomatitis virus envelope. It plays a key role in the virus's ability to infect host cells by facilitating the fusion of the viral envelope with the host cell membrane. Structurally, VSV G is a type Ⅰ transmembrane protein that forms trimers on the viral surface. Its ectodomain contains regions that are important for receptor binding and membrane fusion. The protein undergoes conformational changes during the infection process to mediate these functions. Additionally, VSV G has been widely used in research as a model to study viral entry mechanisms and has applications in the development of viral vectors for gene therapy and vaccines due to its efficient ability to enter cells.
ELISA
Antigen (VSV G (ΔTM) Protein, His-tag, 1ug/ml) is coated overnight at 4°C. Anti-VSV G polyclonal Antibody is added in serial dilutions starting at 1 μg/ml (3-fold, 9 points) and incubated at 37°C for 1 hours. Peroxidase AffiniPure™ Goat Anti-Rabbit IgG (H+L) secondary antibody is added at 37°C for 40 min. Substrate solution is added and developed for 5 minutes at room temperature. Absorbance is measured at 450 nm. Data on the graph represents the percentage of antibody activity calculated by ELISA value.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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