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Rabbit抗原名称
ARL13B分子别名
ADP-ribosylation factor-like protein 13B; ADP-ribosylation factor-like protein 2-like 1 (ARL2-like protein 1); ARL2L1免疫原
Synthetic Peptide细胞定位
Cytoskeleton, Cell membrane, CytoplasmAccession
Q3SXY8克隆号
S-2113-11抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HEK-293, 293T, NCCIT纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200-1:2000 Hu, Ms, Rt ICC 1:500 Ms IF 1:500 Rt
ARL13B, also known as ADP-ribosylation factor-like protein 13B, is a small GTPase belonging to the ADP-ribosylation factor family and the Ras superfamily of GTPases. It is encoded by the ARL13B gene in humans and is predominantly localized in primary cilia. This protein plays a crucial role in cilia formation and maintenance. Mutations in the ARL13B gene can lead to Joubert syndrome, a ciliopathy characterized by brain malformations, polydactyly, and renal cyst formation. ARL13B is also involved in regulating intraflagellar transport and the localization of other proteins within the cilium, such as Smoothened (Smo), which is essential for Sonic hedgehog (Shh) signaling. Additionally, ARL13B has been found to promote the proliferation, migration, and osteogenesis of osteoblasts, suggesting its potential role in bone formation and mechanosensation.
免疫印迹
WB result of ARL13B Recombinant Rabbit mAb
Primary antibody: ARL13B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Lane 4: NCCIT whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 55 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-ARL13B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse brain. Anti-ARL13B antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-ARL13B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-ARL13B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat brain. Anti-ARL13B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in NIH/3T3 cells. Anti-ARL13B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
IF shows positive staining in paraffin-embedded rat brain. Anti-ARL13B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of ARL13B in tumor tissue.
Expression of ARL13B in human tissue.
Expression of ARL13B in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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