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Rabbit抗原名称
ART1分子别名
GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1; ADP-ribosyltransferase C2 and C3 toxin-like 1 (ARTC1); Mono(ADP-ribosyl)transferase 1; CD296免疫原
Recombinant ProteinAccession
P52961克隆号
S-2396-1抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HEK-293, HepG2, MCF7, mouse skeletal muscle, mouse heart纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:1000 Hu, Ms, Rt
ART1 (ADP-ribosyltransferase 1) is a glycosylphosphatidylinositol (GPI)-anchored mono-ADP-ribosyltransferase containing an extracellular catalytic domain that modifies target proteins through ADP-ribosylation, thereby regulating diverse cellular functions. Research has demonstrated that ART1 is highly expressed in various cancers (e.g., colorectal cancer, non-small cell lung cancer, and glioblastoma) and associated with poor prognosis. In the tumor microenvironment, ART1 binds to the P2X7 receptor (P2X7R) on CD8⁺ T cells, inducing T cell death and promoting immune evasion and resistance to immune checkpoint inhibitors (ICIs). Additionally, in colorectal cancer, ART1 activates the AKT/mTOR/STAT3 signaling pathway to drive tumor proliferation and enhances tumor growth under high norepinephrine conditions, which is linked to diabetes-associated colorectal cancer progression. Recent studies have identified that ART1-specific antibodies can reverse its immunosuppressive effects and boost anti-tumor immune responses, positioning ART1 as a promising novel target for cancer immunotherapy.
免疫印迹
WB result of ART1 Recombinant Rabbit mAb
Primary antibody: FOXP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 33 kDaWB result of ART1 Recombinant Rabbit mAb
Primary antibody: FOXP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 39 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-ART1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-ART1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-ART1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-ART1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-ART1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ART1 in tumor tissue.
Expression of ART1 in human tissue.
Expression of ART1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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