FOXP2 Recombinant Rabbit mAb (S-2342-8)

Forkhead box protein P2,CAG repeat protein 44,Trinucleotide repeat-containing gene 10 protein,CAGH44,TNRC10

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6140

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

FOXP2
分子别名

Forkhead box protein P2; CAG repeat protein 44; Trinucleotide repeat-containing gene 10 protein; CAGH44; TNRC10
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

O15409
克隆号

S-2342-8
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms
阳性样本

HEK-293, HeLa, HCT 116, Neuro-2a
预测反应种属
(反应种属缩写表)

NHPr, Xe
纯化方式

Protein A
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:500-1:1000 Hu, Ms
ICC 1:100 Hu
ICFCM 1:200 Hu

应用	稀释度	推荐种属
WB	1:500-1:1000	Hu, Ms
ICC	1:100	Hu
ICFCM	1:200	Hu

背景介绍

FOXP2 protein is a transcription factor that plays a crucial role in the development and functioning of certain neural circuits related to language and speech production. It is encoded by the FOXP2 gene. Mutations in this gene can lead to speech and language disorders. The protein is expressed in various regions of the brain, including the basal ganglia and the cortex. It helps regulate the expression of other genes involved in the formation of neural connections and the development of motor skills necessary for speech. FOXP2 is also found in other species and is believed to be involved in vocalizations and other forms of communication. Its discovery and study have provided valuable insights into the genetic basis of language and the complex mechanisms underlying human communication abilities.

免疫印迹
- WB result of FOXP2 Recombinant Rabbit mAb
  Primary antibody: FOXP2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: HCT 116 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 80 kDa
  Observed MW: 70 kDa
- WB result of FOXP2 Recombinant Rabbit mAb
  Primary antibody: FOXP2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Neuro-2a whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 80 kDa
  Observed MW: 70 kDa
  This blot was developed with high sensitivity substrate
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling FOXP2 antibody at 1/200 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- FOXP2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).