GAD65 Recombinant Rabbit mAb (S-2318-47)

Glutamate decarboxylase 2,65 kDa glutamic acid decarboxylase (GAD-65),Glutamate decarboxylase 65 kDa isoform,GAD2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6139

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

GAD65
分子别名

Glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase (GAD-65); Glutamate decarboxylase 65 kDa isoform; GAD2
免疫原

Synthetic Peptide
细胞定位

Cell membrane, Cytoplasm
Accession

Q05329
克隆号

S-2318-47
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Ms, Rt
阳性样本

mouse brain, mouse cerebellum, rat brain, rat cerebellum
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:10000 Ms, Rt
IHC-P 1:250 Ms, Rt

应用	稀释度	推荐种属
WB	1:1000-1:10000	Ms, Rt
IHC-P	1:250	Ms, Rt

背景介绍

Glutamic acid decarboxylase 65 (GAD65) is an enzyme that synthesizes the inhibitory neurotransmitter γ-aminobutyric acid (GABA) from glutamate, playing a crucial role in the central nervous system. It is one of the two isoforms of glutamic acid decarboxylase, the other being GAD67. GAD65 is particularly notable for its dynamic structure and its role as a major autoantigen in type 1 diabetes (T1D), with autoantibodies against it often present before the clinical onset of the disease. Structurally, GAD65 forms obligate functional dimers and consists of three domains: the N-terminal domain (NTD), the pyridoxal-5'-phosphate (PLP)-binding domain, and the C-terminal domain (CTD). Its catalytic loop (CL) is dynamic, which contrasts with the stable conformation of GAD67's CL.

免疫印迹
- WB result of GAD65 Recombinant Rabbit mAb
  Primary antibody: GAD65 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse brain lysate 20 µg
  Lane 2: mouse cerebellum lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 65 kDa
  Observed MW: 65 kDa
- WB result of GAD65 Recombinant Rabbit mAb
  Primary antibody: GAD65 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat brain lysate 20 µg
  Lane 2: rat cerebellum lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 65 kDa
  Observed MW: 65 kDa
免疫沉淀
免疫组化
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-GAD65 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-GAD65 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-GAD65 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat pancreas. Anti-GAD65 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of GAD65 in mouse & rat tissue.