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Rabbit抗原名称
AP2α分子别名
Transcription factor AP-2-alpha; AP2-alpha; AP-2 transcription factor; Activating enhancer-binding protein 2-alpha; Activator protein 2 (AP-2); AP2TF; TFAP2; TFAP2A免疫原
Synthetic Peptide细胞定位
NucleusAccession
P05549克隆号
S-2376-7抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
SK-BR-3, T-47D, HeLa, 293T纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu, Ms, Rt ICC 1:500 Hu ICFCM 1:50 Hu
AP2α (Activating Protein-2α) is a nuclear transcription factor belonging to the AP-2 family, which specifically binds to DNA and regulates gene expression. It plays a critical role in various biological processes, including embryonic development, cell proliferation, apoptosis, tumor suppression, and immune regulation. The AP2α protein structure consists of an N-terminal proline- and glutamine-rich (P/Q-rich) transactivation domain, a central basic region, and a highly conserved C-terminal helix-span-helix motif responsible for DNA binding and protein dimerization. Studies have shown that AP2α acts as a tumor suppressor in multiple cancers (e.g., gastric, colorectal, and liver cancers) by regulating downstream target genes (such as p21, caspase family members, and EGF receptor) to inhibit tumor cell proliferation and promote apoptosis. Additionally, AP2α is involved in metabolic regulation (e.g., lipid synthesis) and inflammatory responses. For instance, in atherosclerosis, it upregulates IkBα expression via the AMPK signaling pathway to suppress vascular inflammation. In immunity, AP2α has been found to be significantly upregulated (particularly in the liver) in yellow croaker (Larimichthys crocea) in response to Vibrio harveyi infection, indicating its role in innate immune defense. Dysregulated expression or dysfunction of AP2α is associated with various diseases, making it a potential diagnostic biomarker and therapeutic target.
免疫印迹
WB result of AP2α Recombinant Rabbit mAb
Primary antibody: AP2α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: SK-BR-3 whole cell lysate 20 µg
Lane 3: T-47D whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: 293T whole cell lysate 20 µg
Low expression control: HepG2 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 48 kDa
This blot was developed with high sensitivity substrate
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized SK-BR-3 (Human breast adenocarcinoma epithelial cell) labelling AP2α antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti- AP2α antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti- AP2α antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti- AP2α antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- AP2α antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti- AP2α antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti- AP2α antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti- AP2α antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in SKBR-3 cells (top panel) and negative staining in HepG2 cells (below panel). Anti-AP2α antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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