CENPB Recombinant Rabbit mAb (S-1713-209)

Major centromere autoantigen B,Centromere protein B (CENP-B)

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6133

规格

25μl100μl1ml

数量

产品介绍

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产品规格

应用	稀释度	推荐种属
WB	1:1000	Hu
ICC	1:500	Hu

背景介绍

CENPB protein, which stands for centromere protein B, is a crucial component of the centromere, a specialized region on chromosomes. It plays a vital role in ensuring proper chromosome segregation during cell division. CENPB is involved in the formation and maintenance of centromeric chromatin, helping to establish and stabilize the structure of the centromere. This protein also aids in the recruitment of other essential centromere-associated proteins, thereby facilitating the assembly of the kinetochore, a complex protein structure that forms on the centromere and is essential for the attachment of spindle microtubules during mitosis and meiosis. Mutations or abnormalities in CENPB can lead to chromosomal instability and are associated with various genetic disorders and cancers.

免疫印迹
- WB result of CENPB Recombinant Rabbit mAb
  Primary antibody: CENPB Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: A431 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 65 kDa
  Observed MW: 80 kDa
  This blot was developed with high sensitivity substrate
免疫细胞化学
- ICC shows positive staining in A431 cells. Anti-CENPB antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).