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M6PR Recombinant Rabbit mAb (S-2348-14)

Cation-independent mannose-6-phosphate receptor,CI Man-6-P receptor,CI-MPR,300 kDa mannose 6-phosphate receptor (MPR 300),Insulin-like growth factor 2 receptor,Insulin-like growth factor II receptor (IGF-II receptor),M6P/IGF2 receptor (M6P/IGF2R),CD222,MPRI,IGF2R

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6130
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    M6PR

  • 分子别名

    Cation-independent mannose-6-phosphate receptor; CI Man-6-P receptor; CI-MPR; 300 kDa mannose 6-phosphate receptor (MPR 300); Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor (IGF-II receptor); M6P/IGF2 receptor (M6P/IGF2R); CD222; MPRI; IGF2R

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Golgi apparatus membrane

  • Accession

    P11717

  • 克隆号

    S-2348-14

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    293T, HeLa, Jurkat, SK-MEL-28, NIH/3T3, C6

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    WB

    IF ?

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:5000 Hu, Ms, Rt
    IHC-P 1:250 Hu
    IF 1:100 Hu
背景介绍

  • M6PR，which stands for Mannose-6-Phosphate Receptor，is a crucial protein in the cellular processes of eukaryotic organisms. It primarily resides in the Golgi apparatus and endosomes. The M6PR has the ability to recognize and bind to mannose-6-phosphate residues that are present on certain lysosomal enzymes. This binding is essential for the proper sorting and transport of these enzymes. When lysosomal enzymes are synthesized in the endoplasmic reticulum and then transported to the Golgi apparatus，they are tagged with mannose-6-phosphate. The M6PR captures these tagged enzymes in the trans-Golgi network and helps to package them into clathrin-coated vesicles. These vesicles then transport the enzymes to the endosomes，where the M6PR releases the enzymes. The enzymes are subsequently delivered to the lysosomes to carry out their functions in breaking down various biomolecules. In addition to its role in intracellular transport，the M6PR also participates in the endocytosis of extracellular molecules that have been modified with mannose-6-phosphate，contributing to the cell's nutrient uptake and waste processing.

  • 免疫印迹

    • WB result of M6PR Recombinant Rabbit mAb
      Primary antibody: M6PR Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: A549 whole cell lysate 20 µg
      Lane 2: 293T whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Lane 4: Jurkat whole cell lysate 20 µg
      Lane 5: SK-MEL-28 whole cell lysate 20 µg
      Low expression control: A549 whole cell lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 274 kDa
      Observed MW: 310 kDa

    • WB result of M6PR Recombinant Rabbit mAb
      Primary antibody: M6PR Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 274 kDa
      Observed MW: 310 kDa

    • WB result of M6PR Recombinant Rabbit mAb
      Primary antibody: M6PR Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 274 kDa
      Observed MW: 310 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-M6PR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-M6PR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human testis. Anti-M6PR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-M6PR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-M6PR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-M6PR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-M6PR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human pancreatic carcinoma. Anti-M6PR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human testis. Anti-M6PR antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

  • 组织表达图谱

    • Expression of M6PR in tumor tissue.

    • Expression of M6PR in human tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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