ADRP/Perilipin-2 Recombinant Rabbit mAb (S-2210-3)

Perilipin-2,Adipophilin,Adipose differentiation-related protein (ADRP),ADFP,PLIN2

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6128

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

ADRP/Perilipin-2
分子别名

Perilipin-2; Adipophilin; Adipose differentiation-related protein (ADRP); ADFP; PLIN2
免疫原

Synthetic Peptide
细胞定位

Membrane
Accession

Q99541
克隆号

S-2210-3
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms
阳性样本

HepG2, 293T, K562, NIH/3T3, mouse liver
预测反应种属
(反应种属缩写表)

Bv, Pg
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:2000 Hu, Ms
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000-1:2000	Hu, Ms
ICC	1:500	Hu

背景介绍

Adipose Differentiation-Related Protein (ADRP), also known as Perilipin-2 or ADFP, is a 49 kDa lipid droplet surface protein belonging to the PAT family (which includes Perilipin, ADRP, TIP47, S3-12, and OXPAT). It plays a crucial role in lipid droplet formation, stabilization, and metabolic regulation. ADRP is expressed in various cell types, such as hepatic stellate cells, adipocytes, and macrophages, where it modulates lipid storage and release. Studies show that ADRP promotes intracellular lipid accumulation by inhibiting ATGL (adipose triglyceride lipase)-mediated lipolysis while enhancing exogenous fatty acid uptake, thereby influencing lipid droplet size and secretion. Additionally, dysregulated ADRP expression is associated with pathological conditions, including atherosclerosis, non-alcoholic fatty liver disease (NAFLD), and certain cancers. For example, ADRP is highly expressed in foam cells of atherosclerotic plaques, promoting cholesterol ester accumulation, whereas its expression is reduced in the livers of type 2 diabetic patients with fatty liver disease.

免疫印迹
- WB result of ADRP/Perilipin-2 Recombinant Rabbit mAb
  Primary antibody: ADRP/Perilipin-2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: 293T whole cell lysate 20 µg
  Lane 4: K562 whole cell lysate 20 µg
  Low expression control: HeLa whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 48 kDa
- WB result of ADRP/Perilipin-2 Recombinant Rabbit mAb
  Primary antibody: ADRP/Perilipin-2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 48 kDa
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti-ADRP antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).