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宿主来源
Rabbit抗原名称
LFIT3免疫原
Recombinant Protein细胞定位
MitochondrionAccession
Q5T765克隆号
S-1893-45抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
MDA-MB-231, Daudi纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu IHC-P 1:50-1:200 Hu
Interferon-induced protein with tetratricopeptide repeats 3 (IFIT3) is a key player in the immune response, particularly in antiviral defense and cellular signaling regulation. It is a member of the IFIT family and interferon-stimulated genes family, with a structure defined by tetratricopeptide repeats (TPRs) that facilitate protein-protein interactions. IFIT3 plays a crucial role in antiviral innate immunity by recognizing viral RNA and activating immune pathways, leading to antiviral responses. It also modulates the intensity and duration of immune responses, influencing processes like apoptosis and cell survival. In addition to its antiviral functions, IFIT3 is involved in cellular biology changes, including cell proliferation, apoptosis, differentiation, and cancer development. For example, in pancreatic ductal adenocarcinoma, high expression of IFIT3 strengthens the anti-apoptotic ability of cancer cells and increases their resistance to chemotherapeutic drugs.
免疫印迹
WB result of LFIT3 Recombinant Rabbit mAb
Primary antibody: LFIT3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: MDA-MB-231 whole cell lysate 20 µg
Lane 3: Daudi whole cell lysate 20 µg
Negative control: 293T whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-LFIT3 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-LFIT3 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung. Anti-LFIT3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-LFIT3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of LFIT3 in tumor tissue.
Expression of LFIT3 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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