LC3B Recombinant Rabbit mAb (S-1599-142)
Microtubule-associated protein 1 light chain 3 beta,Autophagy-related protein LC3 B,Autophagy-related ubiquitin-like modifier LC3 B,MAP1 light chain 3-like protein 2,Microtubule-associated proteins 1A/1B light chain 3B (MAP1A/MAP1B LC3 B,MAP1A/MAP1B light chain 3 B),MAP1ALC3,MAP1LC3B
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宿主来源
Rabbit抗原名称
LC3B分子别名
Microtubule-associated protein 1 light chain 3 beta; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; Microtubule-associated proteins 1A/1B light chain 3B (MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B); MAP1ALC3; MAP1LC3B免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cytoskeleton, MitochondrionAccession
Q9GZQ8克隆号
S-1599-142抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:200-1:500 Hu, Ms, Rt ICC 1:500 Hu
LC3B protein is a crucial component in the autophagy process. It is a member of the LC3/GABARAP family of proteins. During autophagy, LC3B is cleaved by the enzyme Atg4 to generate the cytosolic form LC3B - I. Then LC3B - I is conjugated to phosphatidylethanolamine to form the membrane - bound form LC3B - II, which is recruited to the autophagosome membrane and plays an important role in the formation and maturation of autophagosomes. The conversion of LC3B - I to LC3B - II is often used as a marker to monitor autophagy activity. LC3B is involved in various cellular processes including the clearance of damaged organelles and protein aggregates, maintaining cellular homeostasis and responding to cellular stress such as nutrient deprivation and hypoxia. Dysregulation of LC3B - related autophagy has been implicated in the pathogenesis of many diseases like neurodegenerative disorders and cancer.
免疫印迹
WB result of LC3B Recombinant Rabbit mAb
Primary antibody: LC3B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 50 μM Chloroquine overnight whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 14 kDa
Observed MW: 14, 16 kDaWB result of LC3B Recombinant Rabbit mAb
Primary antibody: LC3B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 50 μM Chloroquine overnight whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 14 kDa
Observed MW: 14, 15 kDaWB result of LC3B Recombinant Rabbit mAb
Primary antibody: LC3B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 50 μM Chloroquine overnight whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 14 kDa
Observed MW: 14, 15 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-LC3B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-LC3B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-LC3B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-LC3B antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-LC3B antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC analysis of HeLa cells treated with Chloroquine (50μM,24h) (top panel) and untreated HeLa cells (below panel). Anti-LC3B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of LC3B in tumor tissue.
Expression of LC3B in human tissue.
Expression of LC3B in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
引用文献(1)
- α-synuclein triggers NCOA4-FTH1-mediated ferroptosis of oligodendrocyte in multiple system atrophy
Zhenwei Yu, Ehsan Arkin, Yang Li, Marie-Hélène Canron, Marie-Laure Arotcarena, Hugo Jadot, Elio Balpe, Yue Huang, Tao Feng, Wassilios G. Meissner & Jing Zhang
Acta Neuropathologica. 2026 Jan 26 .
影响因子： 9.3
货号：S0B6126产品名称：LC3B Recombinant Rabbit mAb (S-1599-142)
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