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Rabbit抗原名称
TBR2/Eomes分子别名
Eomesodermin homolog; T-box brain protein 2 (T-brain-2; TBR-2); EOMES免疫原
Synthetic Peptide细胞定位
NucleusAccession
O95936克隆号
S-1101-123抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
NK-92, mouse embryo, normal mouse E14 fetal brain, normal rat E14 fetal brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:10000 Hu, Ms, Rt
TBR2/Eomes is a T-box transcription factor that plays crucial roles in various developmental processes. It is preferentially enriched in intermediate progenitors and supports cortical neurogenesis expansion. In the developing cerebral cortex, it is specifically expressed in intermediate progenitors (IPs), which are derived from radial glia progenitors and generate glutamatergic projection neurons. TBR2/Eomes is essential for the differentiation and subtype specification of these neurons, and it regulates the transcriptome by activating or repressing hundreds of direct target genes. Additionally, TBR2/Eomes is involved in the formation and maintenance of certain retinal ganglion cells. It also plays a role in the maintenance of the trophectoderm lineage by sustaining chromatin accessibility and promoting gene regulatory networks.
免疫印迹
WB result of TBR2/Eomes Recombinant Rabbit mAb
Primary antibody: TBR2/Eomes Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: NK-92 whole cell lysate 20 µg
Negative control: THP-1 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 68, 90 kDaWB result of TBR2/Eomes Recombinant Rabbit mAb
Primary antibody: TBR2/Eomes Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Lane 3: mouse embryo lysate 20 µg
Lane 4: normal mouse E14 fetal brain lysate 20 µg
Negative control: mouse liver lysate; mouse spleen lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 70, 95 kDaWB result of TBR2/Eomes Recombinant Rabbit mAb
Primary antibody: TBR2/Eomes Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: normal rat E14 fetal brain lysate 20 µg
Negative control: rat liver lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 70, 100 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,我们对一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定







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