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Rabbit抗原名称
TOX分子别名
Thymocyte selection-associated high mobility group box protein TOX; Thymus high mobility group box protein TOX; KIAA0808免疫原
Recombinant Protein细胞定位
NucleusAccession
O94900克隆号
S-2296-44抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Raji, mouse spleen, mouse thymus, rat spleen, rat thymus纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Hu IHC-P 1:250 Hu
TOX protein is a transcription factor that plays a crucial role in the immune system. It is involved in the regulation of T cell differentiation and function, particularly in the development and maintenance of regulatory T cells (Tregs) which are essential for maintaining immune tolerance and preventing autoimmunity. Additionally, TOX has been implicated in the response of T cells to chronic antigen stimulation, such as in the context of chronic infections or cancer, where it may contribute to T cell exhaustion. Its expression and activity can influence the balance between effector and regulatory T cell populations, thereby having a significant impact on the overall immune response.
免疫印迹
WB result of TOX Recombinant Rabbit mAb
Primary antibody: TOX Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Low expression control: A549 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 60-70 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human thymus. Anti-TOX antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-TOX antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-TOX antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse thymus. Anti-TOX antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-TOX antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-TOX antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of TOX in tumor tissue.
Expression of TOX in human tissue.
Expression of TOX in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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