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宿主来源
Rabbit抗原名称
Rheb分子别名
GTP-binding protein Rheb; Ras homolog enriched in brain; RHEB2; RHEB免疫原
Synthetic Peptide细胞定位
Cytoplasm, Lysosome, Endoplasmic reticulumAccession
Q15382抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu阳性样本
A549, U-87 MG, SH-SY5Y预测反应种属
(反应种属缩写表)Bv, Dr, Ms, Rt纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200 Hu
Rheb protein is a small GTPase that plays a crucial role in regulating cell growth and metabolism. It acts as a key activator of the mTOR (mechanistic target of rapamycin) pathway, which is central to controlling protein synthesis, cell size, and autophagy. When Rheb is bound to GTP, it stimulates mTORC1 activity, promoting anabolic processes that support cell growth and proliferation. Conversely, when it is in its GDP-bound state, mTORC1 is inhibited, allowing cells to shift towards catabolic processes. Rheb is involved in various cellular functions and its dysregulation has been implicated in several diseases, including cancer and neurodegenerative disorders.
免疫印迹
WB result of Rheb Rabbit pAb
Primary antibody: Rheb Rabbit pAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Lane 3: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 20 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Rheb antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Rheb antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Rheb antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Rheb antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Rheb antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Rheb in tumor tissue.
Expression of Rheb in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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