PCK1 Recombinant Rabbit mAb (S-2350-30)

Phosphoenolpyruvate carboxykinase,cytosolic [GTP],PEPCK-C,Serine-protein kinase PCK1,PEPCK1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6115

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

PCK1
分子别名

Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEPCK-C; Serine-protein kinase PCK1; PEPCK1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Endoplasmic reticulum
Accession

P35558
克隆号

S-2350-30
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu
ICFCM 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu
ICFCM	1:50	Hu

背景介绍

PCK1, also known as phosphoenolpyruvate carboxykinase 1, is a crucial enzyme involved in gluconeogenesis and the conversion of oxaloacetate to phosphoenolpyruvate. It plays a significant role in maintaining glucose homeostasis by facilitating the production of glucose from non-carbohydrate sources. This enzyme is primarily expressed in tissues such as the liver and kidneys, where gluconeogenesis predominantly occurs. Abnormal regulation or activity of PCK1 has been implicated in metabolic disorders, including diabetes.

免疫印迹
- WB result of PCK1 Recombinant Rabbit mAb
  Primary antibody: PCK1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: COLO 205 whole cell lysate 20 µg
  Lane 2: HEK-293 whole cell lysate 20 µg
  Lane 3: LNCaP whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 69 kDa
  Observed MW: 69 kDa
- WB result of PCK1 Recombinant Rabbit mAb
  Primary antibody: PCK1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 69 kDa
  Observed MW: 69 kDa
- WB result of PCK1 Recombinant Rabbit mAb
  Primary antibody: PCK1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 69 kDa
  Observed MW: 69 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized A549 (Human lung carcinoma epithelial cell) labeled with PCK1 antibody at 1/50 dilution (1 μg) / (red) compared with a Rabbit monoclonal IgG (black) Isotype Control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
免疫细胞化学
- ICC shows positive staining in A549 cells. Anti-PCK1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).