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Rabbit抗原名称
SOCS3分子别名
Suppressor of cytokine signaling 3; SOCS-3; Cytokine-inducible SH2 protein 3 (CIS-3); STAT-induced STAT inhibitor 3 (SSI-3); CIS3; SSI3免疫原
Recombinant Protein细胞定位
CytoplasmAccession
O14543克隆号
S-2238-34抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
HeLa, Ramos, Jurkat, RAW264.7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms
SOCS3 (Suppressor of Cytokine Signaling 3) is a critical regulator of cytokine signaling pathways. It is an intracellular protein that plays a key role in modulating immune responses and maintaining homeostasis. SOCS3 primarily functions by inhibiting the JAK-STAT signaling pathway, a crucial pathway activated by various cytokines such as interleukins and interferons. By binding to activated JAKs (Janus kinases) and preventing their phosphorylation of downstream targets like STAT proteins, SOCS3 effectively dampens the signaling cascade, thereby controlling the intensity and duration of cytokine-mediated effects. Its expression is often induced by cytokines themselves, creating a negative feedback loop that helps prevent excessive immune activation. Dysregulation of SOCS3 has been implicated in several diseases, including chronic inflammation and autoimmune disorders, highlighting its importance in maintaining proper immune function and overall health.
免疫印迹
WB result of SOCS3 Recombinant Rabbit mAb
Primary antibody: SOCS3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: Ramos whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate; Ramos whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 26 kDa
This blot was developed with high sensitivity substrateWB result of SOCS3 Recombinant Rabbit mAb
Primary antibody: SOCS3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated RAW264.7 whole cell lysate 20 µg
Lane 2: RAW264.7 treated with 100 ng/ml LPS for 6 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 26 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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