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Rabbit抗原名称
SLC40A1/Ferroportin分子别名
Ferroportin; Ferroportin-1; Iron-regulated transporter 1; Solute carrier family 40 member 1Imported; FPN; FPN1; IREG1; SLC11A3免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q9NP59克隆号
S-2172-35抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Caco-2, T-47D, mouse spleen, mouse liver, rat spleen纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt IHC-P 1:500 Hu, Ms, Rt ICC 1:500 Hu
SLC40A1, also known as Ferroportin, is a crucial iron exporter protein that plays a vital role in iron homeostasis. It is primarily located in the plasma membrane of cells involved in iron metabolism, such as enterocytes, hepatocytes, and macrophages. In enterocytes, Ferroportin facilitates the transport of iron absorbed from the diet into the bloodstream. In hepatocytes and macrophages, it helps in the release of stored iron for systemic use. This protein contains multiple transmembrane domains and functions by moving iron ions from the intracellular space to the extracellular environment. Mutations in the SLC40A1 gene can lead to conditions like hemochromatosis, where iron overload occurs due to impaired iron export. Its regulation is also influenced by hepcidin, a hormone that binds to Ferroportin and induces its internalization and degradation, thereby controlling iron release from cells.
免疫印迹
WB result of SLC40A1/Ferroportin Recombinant Rabbit mAb
Primary antibody: SLC40A1/Ferroportin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled A431 whole cell lysate 20 µg
Lane 2: unboiled Caco-2 whole cell lysate 20 µg
Lane 3: unboiled T-47D whole cell lysate 20 µg
Negative control: unboiled A431 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 60-70 kDaWB result of SLC40A1/Ferroportin Recombinant Rabbit mAb
Primary antibody: SLC40A1/Ferroportin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse spleen lysate 20 µg
Lane 2: unboiled mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 65 kDaWB result of SLC40A1/Ferroportin Recombinant Rabbit mAb
Primary antibody: SLC40A1/Ferroportin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 65 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-SLC40A1/Ferroportin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-SLC40A1/Ferroportin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-SLC40A1/Ferroportin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-SLC40A1/Ferroportin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-SLC40A1/Ferroportin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-SLC40A1/Ferroportin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in T47D cells (top panel) and negative staining in A431 cells (below panel). Anti- SLC40A1/Ferroportin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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