SAMHD1 Recombinant Rabbit mAb (S-2401-25)
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1,dNTPase,Dendritic cell-derived IFNG-induced protein (DCIP),Monocyte protein 5 (MOP-5),SAM domain and HD domain-containing protein 1 (hSAMHD1),MOP5
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宿主来源
Rabbit抗原名称
SAMHD1分子别名
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1; dNTPase; Dendritic cell-derived IFNG-induced protein (DCIP); Monocyte protein 5 (MOP-5); SAM domain and HD domain-containing protein 1 (hSAMHD1); MOP5免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q9Y3Z3克隆号
S-2401-25抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Daudi, Ramos纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:5000 Hu IHC-P 1:100 Hu
SAMHD1 (Sterile Alpha Motif and HD Domain-Containing Protein 1) is a protein that plays a crucial role in cellular processes. It is involved in the regulation of dNTP (deoxyribonucleoside triphosphate) levels within cells, which is essential for DNA synthesis and repair. SAMHD1 acts as a dNTP triphosphohydrolase, helping to maintain a balanced pool of dNTPs to prevent excessive DNA replication and genomic instability. Additionally, it has been implicated in innate immune responses, particularly in restricting the replication of certain viruses, such as HIV-1, by limiting the availability of dNTPs required for viral reverse transcription. Its dysfunction has been associated with various diseases, including Aicardi-Goutières syndrome, an autoimmune disorder characterized by chronic inflammation and neurological damage.
免疫印迹
WB result of SAMHD1 Recombinant Rabbit mAb
Primary antibody: SAMHD1 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: HCC827 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: Daudi whole cell lysate 20 µg
Lane 4: Ramos whole cell lysate 20 µg
Negative control: HCC827 whole cell lysate; Jurkat whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 73 kDa
Observed MW: 70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human spleen. Anti-SAMHD1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-SAMHD1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SAMHD1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-SAMHD1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of SAMHD1 in tumor tissue.
Expression of SAMHD1 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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