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ARNT Recombinant Rabbit mAb (S-2263-43)

Aryl hydrocarbon receptor nuclear translocator,ARNT protein,Class E basic helix-loop-helix protein 2 (bHLHe2),Dioxin receptor,nuclear translocator,Hypoxia-inducible factor 1-beta (HIF-1-beta,HIF1-beta),BHLHE2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6110

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

ARNT
分子别名

Aryl hydrocarbon receptor nuclear translocator; ARNT protein; Class E basic helix-loop-helix protein 2 (bHLHe2); Dioxin receptor; nuclear translocator; Hypoxia-inducible factor 1-beta (HIF-1-beta; HIF1-beta); BHLHE2
免疫原

Recombinant Protein
细胞定位

Nucleus
Accession

P27540
克隆号

S-2263-43
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HepG2, MDA-MB-231, MCF7, HeLa, 293T, NIH/3T3, RAW264.7, PC-12
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:10000 Hu, Ms, Rt
ICC 1:500 Hu
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
WB	1:1000-1:10000	Hu, Ms, Rt
ICC	1:500	Hu
ChIP	1:20-1:50	Hu

背景介绍

ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator) protein is a crucial transcription factor involved in various cellular processes. It forms heterodimers with other proteins such as the aryl hydrocarbon receptor (AhR), playing a key role in the regulation of gene expression in response to environmental toxins and endogenous signals. ARNT is essential for the proper functioning of the AhR pathway, which is involved in xenobiotic metabolism, cell differentiation, and immune response modulation. Additionally, ARNT has been implicated in developmental processes and maintaining cellular homeostasis. Dysregulation of ARNT function has been associated with several pathological conditions, highlighting its importance in both normal physiology and disease.

免疫印迹
- WB result of ARNT Recombinant Rabbit mAb
  Primary antibody: ARNT Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HepG2 whole cell lysate 20 µg
  Lane 2: MDA-MB-231 whole cell lysate 20 µg
  Lane 3: MCF7 whole cell lysate 20 µg
  Lane 4: HeLa whole cell lysate 20 µg
  Lane 5: 293T whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 87 kDa
  Observed MW: 85 kDa
- WB result of ARNT Recombinant Rabbit mAb
  Primary antibody: ARNT Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: RAW264.7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 87 kDa
  Observed MW: 85 kDa
- WB result of ARNT Recombinant Rabbit mAb
  Primary antibody: ARNT Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 87 kDa
  Observed MW: 85 kDa
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- ARNT antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HepG2 cells cross-linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used ARNT Recombinant Rabbit mAb (S-2263-43) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post -immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of CYP1A1, NFE2L2 and SAT-α in ARNT Recombinant
  Rabbit mAb (S-2263-43)-immunoprecipitated sample.