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Rabbit抗原名称
AIFM2/FSP1分子别名
Ferroptosis suppressor protein 1; Apoptosis-inducing factor homologous mitochondrion-associated inducer of death (AMID); p53-responsive gene 3 protein; AMID; PRG3免疫原
Synthetic Peptide细胞定位
Nucleus, Mitochondrion, Cell membrane, CytoplasmAccession
Q9BRQ8克隆号
S-2271-67抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
HeLa, MCF7, A172, NIH/3T3, mouse kidney, mouse liver预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:200 Hu, Ms
AIFM2, also known as ferroptosis suppressor protein 1 (FSP1), apoptosis-inducing factor-homologous mitochondrion-associated inducer of death (AMID), or p53-responsive gene 3 (PRG3), is a protein encoded by the AIFM2 gene in humans. It is a flavoprotein oxidoreductase that plays a crucial role in regulating ferroptosis, a form of cell death characterized by iron-dependent lipid peroxidation. FSP1 primarily localizes to the plasma membrane and lipid droplets, and it suppresses ferroptosis by reducing ubiquinone (CoQ10) to ubiquinol (CoQ10-H2) using NAD(P)H as a cofactor, thereby preventing lipid peroxidation. This protein is also involved in the reduction of other molecules, such as vitamin K, and has been implicated in tumorigenesis and resistance to therapy in certain cancer models.
免疫印迹
WB result of AIFM2/FSP1 Recombinant Rabbit mAb
Primary antibody: AIFM2/FSP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: A172 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 39 kDaWB result of AIFM2/FSP1 Recombinant Rabbit mAb
Primary antibody: AIFM2/FSP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Lane 3: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 39 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-AIFM2/FSP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-AIFM2/FSP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-AIFM2/FSP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-AIFM2/FSP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of AIFM2/FSP1 in tumor tissue.
Expression of AIFM2/FSP1 in human tissue.
Expression of AIFM2/FSP1 in mouse tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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