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ACLY/ATP citrate lyase Recombinant Rabbit mAb (S-2025-32)

ATP-citrate synthase,ATP-citrate (pro-S-)-lyase (ACL),Citrate cleavage enzyme

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6108
规格
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产品介绍

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    ACLY/ATP citrate lyase

  • 分子别名

    ATP-citrate synthase; ATP-citrate (pro-S-)-lyase (ACL); Citrate cleavage enzyme

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm

  • Accession

    P53396

  • 克隆号

    S-2025-32

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, MCF7, HepG2, Jurkat, 293T, NIH/3T3, mouse liver, C6, rat liver

  • 预测反应种属
    (反应种属缩写表)

    Sh, Bv

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ICFCM

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:5000 Hu, Ms, Rt
    IHC-P 1:1000 Hu, Ms, Rt
    ICC 1:500 Hu
    ICFCM 1:500 Hu
背景介绍

  • ACLY, also known as ATP citrate lyase, is a crucial enzyme involved in cellular metabolism. It catalyzes the conversion of citrate and ATP to oxaloacetate, acetyl-CoA, and ADP, playing a key role in linking glycolysis and lipid metabolism. ACLY is predominantly located in the cytoplasm and is essential for the production of acetyl-CoA, which serves as a vital precursor for fatty acid synthesis and cholesterol biosynthesis. Its activity is tightly regulated by various factors, including insulin and AMPK, reflecting its importance in maintaining metabolic homeostasis. Abnormal expression or activity of ACLY has been implicated in several diseases, such as cancer and metabolic disorders, making it an attractive target for therapeutic interventions.

  • 免疫印迹

    • WB result of ACLY/ATP citrate lyase Recombinant Rabbit mAb
      Primary antibody: ACLY/ATP citrate lyase Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: MCF7 whole cell lysate 20 µg
      Lane 3: HepG2 whole cell lysate 20 µg
      Lane 4: Jurkat whole cell lysate 20 µg
      Lane 5: 293T whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 121 kDa
      Observed MW: 115 kDa

    • WB result of ACLY/ATP citrate lyase Recombinant Rabbit mAb
      Primary antibody: ACLY/ATP citrate lyase Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: mouse liver lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 121 kDa
      Observed MW: 115 kDa

    • WB result of ACLY/ATP citrate lyase Recombinant Rabbit mAb
      Primary antibody: ACLY/ATP citrate lyase Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Lane 2: rat liver lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 121 kDa
      Observed MW: 115 kDa

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling ACLY/ATP citrate lyase antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse stomach. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat testis. Anti-ACLY/ATP citrate lyase antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-ACLY/ATP citrate lyase antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of ACLY/ATP citrate lyase in tumor tissue.

    • Expression of ACLY/ATP citrate lyase in human tissue.

    • Expression of ACLY/ATP citrate lyase in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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