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Rabbit抗原名称
14-3-3 sigma分子别名
14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin; HME1; SFN免疫原
Synthetic Peptide细胞定位
Secreted, Cytoplasm, NucleusAccession
P31947克隆号
S-2017-59抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
HeLa, A431, MCF7, Mouse colon纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms IHC-P 1:2000 Hu ICC 1:500 Hu
The 14-3-3 sigma protein, also known as stratifin, is a member of the 14-3-3 protein family that plays a crucial role in cell cycle regulation. It functions primarily to inhibit the progression of cells from the G2 phase to the M phase by preventing the activation of cyclin-dependent kinases. This protein is highly expressed in various tissues and is involved in maintaining genomic stability. Additionally, 14-3-3 sigma has been implicated in several cellular processes, including apoptosis and signal transduction pathways. Dysregulation of 14-3-3 sigma has been associated with certain cancers and other diseases, making it a potential target for therapeutic interventions.
免疫印迹
WB result of 14-3-3 sigma Recombinant Rabbit mAb
Primary antibody: 14-3-3 sigma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 28 kDaWB result of 14-3-3 sigma Recombinant Rabbit mAb
Primary antibody: 14-3-3 sigma Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse colon lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 28 kDa
免疫沉淀
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-14-3-3 sigma antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-14-3-3 sigma antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-14-3-3 sigma antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human transitional cell carcinoma. Anti-14-3-3 sigma antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-14-3-3 sigma antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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