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Rabbit抗原名称
HuR/ELAVL1分子别名
ELAV-like protein 1; Hu-antigen R (HuR)免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
Q15717克隆号
S-2357-20抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HEK-293, Jurkat, MCF7, HeLa, RAW264.7, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:10000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt ICC 1:500 Hu, Ms, Rt
HuR/ELAVL1 protein, also known as human antigen R, is a ubiquitously expressed RNA-binding protein encoded by the ELAVL1 gene. It is a member of the ELAV family of proteins and contains three RNA recognition motifs (RRMs) that enable it to bind to specific RNA sequences, particularly AU-rich elements (AREs) in the 3' untranslated regions (3'UTRs) of mRNAs. This binding stabilizes the mRNA, protecting it from degradation and enhancing its translation, thereby regulating gene expression. HuR plays crucial roles in various cellular processes, including cell differentiation, stress response, and tumorigenesis. Its dysregulation has been implicated in multiple diseases, such as cancer, cardiovascular diseases, and neurodegenerative disorders.
免疫印迹
WB result of HuR/ELAVL1 Recombinant Rabbit mAb
Primary antibody: HuR/ELAVL1 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 34 kDaWB result of HuR/ELAVL1 Recombinant Rabbit mAb
Primary antibody: HuR/ELAVL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 34 kDaWB result of HuR/ELAVL1 Recombinant Rabbit mAb
Primary antibody: HuR/ELAVL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 34 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-HuR/ELAVL1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-HuR/ELAVL1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-HuR/ELAVL1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-HuR/ELAVL1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-HuR/ELAVL1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-HuR/ELAVL1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HEK293 cells. Anti-HuR/ELAVL1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in HEK293 cells. Anti-HuR/ELAVL1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of HuR/ELAVL1 in tumor tissue.
Expression of HuR/ELAVL1 in human tissue.
Expression of HuR/ELAVL1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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