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产品中心 蛋白翻译后修饰(PTM)抗体 位点特异性修饰抗体

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Histone H3 (tri methyl K4) Recombinant Rabbit mAb (S-1602-21)

H3K4me3

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6104
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 分子别名

    H3K4me3

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P68431

  • 克隆号

    S-1602-21

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    甲基化

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, NIH/3T3, C6

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ChIP

    IHC-P ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    Dot Blot 1:1000
    WB 1:1000-1:5000 Hu, Ms, Rt
    IHC-P 1:5000 Hu, Ms, Rt
    ChIP 1:50
背景介绍

  • Histone H3 (tri-methyl K4) is a chromatin-associated protein that has been modified by the addition of three methyl groups to the lysine residue at position 4. This post-translational modification is typically associated with active transcription, as it creates a binding site for chromatin remodelers and transcription factors, thereby promoting an open chromatin structure that facilitates gene expression.

  • 免疫印迹

    • WB result of Histone H3 (tri methyl K4) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (tri methyl K4) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

    • WB result of Histone H3 (tri methyl K4) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (tri methyl K4) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

    • WB result of Histone H3 (tri methyl K4) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (tri methyl K4) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

  • 斑点杂交

    • Dot blot result of Histone H3 (tri methyl K4) Recombinant Rabbit mAb
      Lane 1: H3K4me3 modified peptide
      Lane 2: H3K4 unmodified peptide
      Lane 3: H3K4me1 modified peptide
      Lane 4: H3K4me2 modified peptide
      Primary antibody: Histone H3 (tri methyl K4) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-Histone H3 (tri methyl K4) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon. Anti-Histone H3 (tri methyl K4) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Histone H3 (tri methyl K4) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human squamous cell carcinoma. Anti-Histone H3 (tri methyl K4) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse colon. Anti-Histone H3 (tri methyl K4) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat colon. Anti-Histone H3 (tri methyl K4) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • ChIP

    • Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (tri methyl K4)
      Recombinant Rabbit mAb (S-1602-21) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
      Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
      qPCR (%input: immunoprecipitated DNA/input DNA) showed the enrichment of RPL30, GAPDH, MYOD1,
      AFM, SAT-α and SAT-2 in Histone H3 (tri methyl K4) Recombinant Rabbit mAb (S-1602-21)-immunoprecipitated
      sample.

  • 组织表达图谱

    • Expression of Histone H3 (tri methyl K4) in tumor tissue.

    • Expression of Histone H3 (tri methyl K4) in human tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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