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Rabbit抗原名称
HADHA分子别名
Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase (MLCL AT); TP-alpha; HADH免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
P40939克隆号
S-2345-8抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
HeLa, Jurkat, HepG2, 293T, NIH/3T3, mouse heart, mouse kidney, PC-12, rat heart, rat kidney预测反应种属
(反应种属缩写表)Pg纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms, Rt IHC-P 1:200 Hu, Ms ICC 1:500 Hu, Ms
HADHA, which stands for hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex alpha subunit, is a crucial protein involved in the mitochondrial beta-oxidation of fatty acids. It is a part of the trifunctional protein complex that catalyzes three sequential steps in the breakdown of long-chain fatty acids into acetyl-CoA, a key molecule in cellular energy production. This protein is essential for the proper metabolism of fats and is particularly important in tissues with high energy demands such as the liver and heart. Mutations in the HADHA gene can lead to severe metabolic disorders, including long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency, which can cause symptoms ranging from hypoglycemia and cardiomyopathy to more severe neurological complications, highlighting its critical role in maintaining metabolic homeostasis.
免疫印迹
WB result of HADHA Recombinant Rabbit mAb
Primary antibody: HADHA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 75 kDaWB result of HADHA Recombinant Rabbit mAb
Primary antibody: HADHA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Lane 3: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 80 kDaWB result of HADHA Recombinant Rabbit mAb
Primary antibody: HADHA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat heart lysate 20 µg
Lane 3: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 80 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-HADHA antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-HADHA antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-HADHA antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-HADHA antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human squamous cell carcinoma. Anti-HADHA antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-HADHA antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- HADHA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- HADHA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of HADHA in tumor tissue.
Expression of HADHA in human tissue.
Expression of HADHA in mouse tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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