Phospho-Stat6 (Tyr641) Recombinant Rabbit mAb (S-1440-135)

Signal transducer and transcription activator 6,Stat6

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6101

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-Stat6 (Tyr641)
分子别名

Signal transducer and transcription activator 6; Stat6
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

P52633
克隆号

S-1440-135
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Rt
ICC 1:100 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Rt
ICC	1:100	Hu

背景介绍

Phospho-Stat6 (Tyr641) protein is a phosphorylated form of the Signal Transducer and Activator of Transcription 6 (Stat6) protein, specifically phosphorylated at tyrosine residue 641. Stat6 is a key transcription factor involved in mediating the cellular response to cytokines, particularly interleukin-4 (IL-4) and interleukin-13 (IL-13), which are crucial for the development and function of Th2 cells and the regulation of allergic responses and immune tolerance. When phosphorylated at Tyr641, Stat6 becomes activated and translocates to the nucleus, where it binds to specific DNA sequences to regulate the expression of genes involved in immune cell differentiation, cytokine production, and the development of allergic inflammation. This phosphorylation event is critical for the downstream signaling cascade initiated by IL-4 and IL-13 receptor activation, making phospho-Stat6 (Tyr641) a key biomarker for studying Th2-mediated immune responses and potential therapeutic target for allergic and immune-related diseases.

免疫印迹
- WB result of Phospho-Stat6 (Tyr641) Recombinant Rabbit mAb
  Primary antibody: Phospho-Stat6 (Tyr641) Recombinant Rabbit mAb at 1/1000 dilution
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Lane 1: untreated Daudi whole cell lysate 10 µg
  Lane 2: Daudi starved for 24 hours, then treated with 100ng/ml IL-4 for 15 minutes whole cell lysate 10 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 93 kDa
  Observed MW: 75, 85, 105 kDa
- WB result of Phospho-Stat6 (Tyr641) Recombinant Rabbit mAb
  Primary antibody: Phospho-Stat6 (Tyr641) Recombinant Rabbit mAb at 1/1000 dilution
  Blocking/Diluting buffer and concentration: 5% NFDM/TBST
  Lane 1: untreated Rat PBMC whole cell lysate 20 µg
  Lane 2: Rat PBMC treated with 50ng/ml rIL-4 for 6 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 93 kDa
  Observed MW: 70, 85, 105 kDa
免疫细胞化学
- ICC analysis of Daudi cells starved for 24h then treated with IL-4 (100ng/ml, 15min) (top panel) and untreated Daudi cells (below panel). Anti- Phospho-STAT6 (Tyr641) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).