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Rabbit抗原名称
CNOT1分子别名
CCR4-NOT transcription complex subunit 1; CCR4-associated factor 1; Negative regulator of transcription subunit 1 homolog (NOT1H; hNOT1); CDC39; KIAA1007; NOT1免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
A5YKK6克隆号
S-2273-65抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
293T, HeLa, Jurkat, NIH/3T3预测反应种属
(反应种属缩写表)Zf, Xe纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms ICC 1:50 Hu, Ms
CNOT1 (CCR4-NOT transcription complex subunit 1) is a crucial protein encoded by the CNOT1 gene and serves as the central scaffold for the CCR4-NOT complex, which is a master regulator of gene expression involved in mRNA deadenylation, transcriptional regulation, and protein ubiquitination. This complex plays a significant role in various cellular processes, including mRNA degradation, DNA repair, and circadian rhythm regulation. CNOT1 itself does not possess catalytic activity but is essential for maintaining the integrity of the CCR4-NOT complex and binding to other subunits and RNA-binding proteins. It is also implicated in neurodevelopmental disorders, as de novo variants in CNOT1 have been associated with intellectual disability, seizures, and other developmental issues.
免疫印迹
WB result of CNOT1 Recombinant Rabbit mAb
Primary antibody: CNOT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 267 kDa
Observed MW: 270 kDaWB result of CNOT1 Recombinant Rabbit mAb
Primary antibody: CNOT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 267 kDa
Observed MW: 270 kDa
免疫细胞化学
ICC shows positive staining in 293T cells. Anti-CNOT1 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-CNOT1 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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