beta Galactosidase/GLB1 Recombinant Rabbit mAb (S-2295-7)

Beta-galactosidase,Acid beta-galactosidase (Lactase),Elastin receptor 1,ELNR1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6098

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

beta Galactosidase/GLB1
分子别名

Beta-galactosidase; Acid beta-galactosidase (Lactase); Elastin receptor 1; ELNR1
免疫原

Recombinant Protein
细胞定位

Lysosome, Cytoplasm
Accession

P16278
克隆号

S-2295-7
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

SK-BR-3, MCF7, Saos-2, U-87 MG, SH-SY5Y
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
IHC-P 1:500 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu
IHC-P	1:500	Hu, Ms, Rt

背景介绍

Beta-galactosidase (GLB1) is an essential lysosomal enzyme responsible for the hydrolysis of terminal galactose residues in glycolipids and glycoproteins. This enzyme plays a crucial role in the degradation and recycling of complex molecules within cells. Deficiencies or mutations in the GLB1 gene can lead to the accumulation of undigested substrates in lysosomes, resulting in severe genetic disorders such as GM1 gangliosidosis and Morquio B syndrome. These conditions are characterized by progressive neurological and skeletal abnormalities due to the impaired breakdown of specific glycosphingolipids and glycoproteins.

免疫印迹
- WB result of beta Galactosidase/GLB1 Recombinant Rabbit mAb
  Primary antibody: beta Galactosidase/GLB1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Daudi whole cell lysate 20 µg
  Lane 2: SK-BR-3 whole cell lysate 20 µg
  Lane 3: MCF7 whole cell lysate 20 µg
  Lane 4: Saos-2 whole cell lysate 20 µg
  Lane 5: U-87 MG whole cell lysate 20 µg
  Lane 6: SH-SY5Y whole cell lysate 20 µg
  Low expression control: Daudi whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 76 kDa
  Observed MW: 65 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-beta Galactosidase/GLB1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human liver. Anti-beta Galactosidase/GLB1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-beta Galactosidase/GLB1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-beta Galactosidase/GLB1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-beta Galactosidase/GLB1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-beta Galactosidase/GLB1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
组织表达图谱
- Expression of beta Galactosidase/GLB1 in tumor tissue.
- Expression of beta Galactosidase/GLB1 in human tissue.