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Rabbit抗原名称
Phospho-Vimentin (Ser56)分子别名
Vimentin; VIM免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cytoskeleton, Cell membraneAccession
P08670克隆号
S-2135-5抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu预测反应种属
(反应种属缩写表)AfGrMk, Cz, Pg, Dg纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000 Hu
Phospho-Vimentin (Ser56) is a phosphorylated form of the vimentin protein, specifically at serine residue 56. Vimentin is a type of intermediate filament protein that plays a crucial role in maintaining cell integrity and providing mechanical support to cells. When phosphorylated at Ser56, it often indicates involvement in cellular processes such as cell migration, adhesion, and the epithelial-mesenchymal transition (EMT). This phosphorylation can be a key marker in various biological contexts, including cancer progression, where it may be associated with the enhanced motility and invasive potential of tumor cells.
免疫印迹
WB result of Phospho-Vimentin (Ser56) Recombinant Rabbit mAb
Primary antibody: Phospho-Vimentin (Ser56) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 nM Paclitaxel for 20 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 57 kDa
斑点杂交
Dot blot result of Phospho-Vimentin (Ser56) Recombinant Rabbit mAb
Lane 1: Vimentin (Ser56) phospho peptide
Lane 2: Vimentin unmodified peptide
Primary antibody: Phospho-Vimentin (Ser56) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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