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Rabbit抗原名称
PRAME分子别名
Melanoma antigen preferentially expressed in tumors; Opa-interacting protein 4 (OIP-4); Preferentially expressed antigen of melanoma; MAPE; OIP4免疫原
Recombinant Protein细胞定位
Nucleus, Cell membrane, CytoplasmAccession
P78395克隆号
S-1876-173抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
A375, U-2 OS, K562, SK-MEL-2纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu ICC 1:500 Hu
PRAME (Preferentially Expressed Antigen of Melanoma) is a protein encoded by the PRAME gene, belonging to the cancer-testis antigen family. It is predominantly expressed in various cancers, including melanoma, breast cancer, lung cancer, and leukemia, but is largely absent in normal tissues except for testis, ovary, placenta, and a few others. PRAME can inhibit retinoic acid signaling, thereby promoting cell proliferation and contributing to tumorigenesis. Due to its restricted expression pattern, PRAME is considered a promising target for cancer immunotherapy, with ongoing research exploring PRAME-specific vaccines and cellular immunotherapies.
免疫印迹
WB result of PRAME Recombinant Rabbit mAb
Primary antibody: PRAME Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A375 whole cell lysate 20 µg
Lane 2: U-2 OS whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: SK-MEL-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 52 kDa
免疫细胞化学
ICC shows positive staining in K562 cells. Anti- PRAME antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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