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Rabbit抗原名称
PHGDH分子别名
D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; PGDH3免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
O43175抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, MCF7, NIH/3T3, mouse brain, C6预测反应种属
(反应种属缩写表)Bv, CyMk, Cz, Or, Pg纯化方式
Immunogen Affinity浓度
Lot specific* (generally 0.5 to 2 mg/ml)标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt
PHGDH (Phosphoglycerate Dehydrogenase) protein is a key enzyme involved in the metabolic pathway that converts 3-phosphoglycerate to phosphohydroxypyruvate. It plays a crucial role in serine biosynthesis, which is essential for various cellular processes including the production of nucleotides, lipids, and antioxidants. Abnormal expression of PHGDH has been implicated in several diseases, particularly in cancer, where its upregulation can promote tumor growth and survival by supporting biosynthetic demands and redox homeostasis. Additionally, PHGDH has been identified as a potential therapeutic target in certain cancer types due to its role in maintaining cancer cell metabolism.
免疫印迹
WB result of PHGDH Rabbit pAb
Primary antibody: PHGDH Rabbit pAb at 1/1000 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Negative control: Daudi whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 57 kDaWB result of PHGDH Rabbit pAb
Primary antibody: PHGDH Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 57 kDaWB result of PHGDH Rabbit pAb
Primary antibody: PHGDH Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 57 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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