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Rabbit抗原名称
MYG1分子别名
MYG1 exonuclease; C12orf10免疫原
Synthetic Peptide细胞定位
Nucleus, MitochondrionAccession
Q9HB07克隆号
S-2248-25抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
SW480, LoVo, SW620, HCT 116纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200 Hu, Ms, Rt ICC 1:500 Hu
MYG1 protein, also known as Melanocyte Proliferating Gene 1, is a dual-localized 3′-5′ RNA exonuclease found in both the nucleus and mitochondria. It plays a crucial role in RNA processing, specifically in the maturation of ribosomal and messenger RNA in mitochondria, which is essential for mitochondrial function and cellular respiration. MYG1 is involved in coordinating nuclear and mitochondrial translation programs, acting as a key mediator of nucleo-mitochondrial communication. In addition to its role in normal cellular function, MYG1 has been implicated in various pathological conditions. For instance, it is upregulated in colorectal cancer, where it promotes glycolysis and tumor progression through mechanisms involving the AMPK/mTOR signaling pathway. MYG1 is also associated with vitiligo, a skin disorder, and may be involved in antiviral responses.
免疫印迹
WB result of MYG1 Recombinant Rabbit mAb
Primary antibody: MYG1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 20 µg
Lane 2: SW480 whole cell lysate 20 µg
Lane 3: LoVo whole cell lysate 20 µg
Lane 4: SW620 whole cell lysate 20 µg
Lane 5: HCT 116 whole cell lysate 20 µg
Low expression control: HT-29 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 38 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-MYG1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-MYG1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-MYG1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-MYG1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in SW480 cells. Anti-MYG1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of MYG1 in tumor tissue.
Expression of MYG1 in human tissue.
Expression of MYG1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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