MFN1 Recombinant Rabbit mAb (S-2230-2)

Mitofusin-1,Fzo homolog,Transmembrane GTPase MFN1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6091

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

MFN1
分子别名

Mitofusin-1; Fzo homolog; Transmembrane GTPase MFN1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Mitochondrion
Accession

Q8IWA4
克隆号

S-2230-2
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms
阳性样本

HeLa, PC-3, MDA-MB-231, T-47D, NIH/3T3
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms
IHC-P 1:200 Hu
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms
IHC-P	1:200	Hu
ICC	1:500	Hu

背景介绍

The MFN1 protein, also known as Mitofusin 1, is a key regulator of mitochondrial fusion. It is a member of the dynamin-related GTPase family and is predominantly localized on the outer mitochondrial membrane. MFN1 plays a crucial role in maintaining mitochondrial morphology and function by mediating the fusion of mitochondrial outer membranes, which helps in the exchange of mitochondrial contents and the maintenance of mitochondrial DNA integrity. Dysregulation of MFN1 has been implicated in various diseases, including neurodegenerative disorders such as Parkinson's disease and Charcot-Marie-Tooth disease type 2A, where impaired mitochondrial dynamics contribute to cellular dysfunction and neuronal degeneration.

免疫印迹
- WB result of MFN1 Recombinant Rabbit mAb
  Primary antibody: MFN1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: PC-3 whole cell lysate 20 µg
  Lane 3: MDA-MB-231 whole cell lysate 20 µg
  Lane 4: T-47D whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 84 kDa
  Observed MW: 85 kDa
- WB result of MFN1 Recombinant Rabbit mAb
  Primary antibody: MFN1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 84 kDa
  Observed MW: 85 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-MFN1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-MFN1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of MFN1 in human tissue.