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Rabbit抗原名称
Gli2分子别名
Zinc finger protein GLI2; GLI family zinc finger protein 2Imported; Tax helper protein; THP; GLI2免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P10070克隆号
S-2193-2抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HepG2, 293T, K562纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu ICC 1:100 Hu
Gli2 protein is a crucial transcription factor in the Hedgehog signaling pathway, playing a significant role in embryonic development and tissue homeostasis. It is involved in regulating cell proliferation, differentiation, and survival in various tissues. Gli2 acts as both an activator and repressor of gene expression, depending on the context and the presence of Hedgehog ligands. In the absence of signaling, Gli2 is processed into a repressor form that inhibits target gene transcription. However, when Hedgehog ligands bind to their receptors, Gli2 is converted into an activator form that promotes the expression of genes essential for proper development and tissue repair. Dysregulation of Gli2 activity has been implicated in various diseases, including cancer and developmental disorders, making it an important target for therapeutic interventions.
免疫印迹
WB result of Gli2 Recombinant Rabbit mAb
Primary antibody: Gli2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 167 kDa
Observed MW: 190 kDa
This blot was developed with high sensitivity substrate
免疫细胞化学
ICC shows positive staining in 293T cells. Anti-Gli2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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