ECM1 Recombinant Rabbit mAb (S-2235-35)

Extracellular matrix protein 1,Secretory component p85,Ecm1

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6088

规格

25μl100μl1ml

数量

产品介绍

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产品规格

应用	稀释度	推荐种属
WB	1:1000	Ms
ICC	1:500	Ms

背景介绍

ECM1 protein, or extracellular matrix protein 1, is a glycoprotein predominantly expressed in the extracellular matrix of various tissues. It plays a crucial role in cell adhesion, proliferation, and differentiation. ECM1 is highly expressed in tissues such as skin, placenta, and blood vessels. Its expression is regulated by several growth factors and hormones. Abnormal expression of ECM1 has been implicated in various pathological conditions, including cancer progression, where it may contribute to tumor growth and metastasis by modulating the tumor microenvironment. Additionally, ECM1 is involved in the development and maintenance of normal tissue architecture and has potential as a biomarker for certain diseases due to its specific expression patterns.

免疫印迹
- WB result of ECM1 Recombinant Rabbit mAb
  Primary antibody: ECM1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C2C12 whole cell lysate 20 µg
  Lane 2: NIH/3T3 whole cell lysate 20 µg
  Lane 3: mouse liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 63 kDa
  Observed MW: 48, 85 kDa
免疫细胞化学
- ICC shows positive staining in NIH/3T3 cells. Anti- ECM1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).