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Rabbit抗原名称
ZO-1分子别名
Tight junction protein 1; Tight junction protein ZO-1; Zona occludens protein 1; Zonula occludens protein 1; ZO1; TJP1免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
Q07157克隆号
S-2188-12抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:250-1:1000 Hu, Ms
The ZO-1 protein is a crucial component of tight junctions, which are essential for maintaining cell polarity and regulating the passage of substances across epithelial and endothelial cell layers. ZO-1, also known as zonula occludens-1, is a peripheral membrane protein that links transmembrane proteins of tight junctions to the actin cytoskeleton. It plays a significant role in the assembly and maintenance of tight junctions, thereby contributing to the barrier function of tissues. Additionally, ZO-1 is involved in various cellular processes such as cell signaling and proliferation, and its dysregulation has been implicated in several pathological conditions including cancer and inflammatory diseases.
免疫组化
IHC shows positive staining in paraffin-embedded human stomach. Anti-ZO-1 Receptor antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-ZO-1 Receptor antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ZO-1 Receptor antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-ZO-1 Receptor antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-ZO-1 Receptor antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ZO-1 Receptor in tumor tissue.
Expression of ZO-1 Receptor in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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