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Rabbit抗原名称
SORT1分子别名
Sortilin; 100 kDa NT receptor; Glycoprotein 95 (Gp95); Neurotensin receptor 3 (NT3; NTR3)免疫原
Recombinant Protein细胞定位
Nucleus membrane, Cell membrane, Endoplasmic reticulum membraneAccession
Q99523克隆号
S-2233-47抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
SH-SY5Y, PANC-1, SK-BR-3, Neruo-2a, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt ICC 1:500 Ms
Sortilin 1 (SORT1) is a type I transmembrane glycoprotein encoded by the SORT1 gene, belonging to the vacuolar protein sorting 10 protein (Vps10p) family of sorting receptors. It is ubiquitously expressed in many tissues, with particularly high levels in the central nervous system. SORT1 plays a critical role in intracellular protein transport between the Golgi apparatus, endosomes, lysosomes, and plasma membrane, and is involved in various biological processes such as glucose and lipid metabolism, neural development, and cell death. The Vps10p domain of SORT1 forms a ten-bladed beta-propeller structure with multiple ligand binding sites, allowing it to bind over 50 proteins. SORT1 is also implicated in several diseases, including hypertension, atherosclerosis, coronary artery disease, Alzheimer’s disease, and cancer. Additionally, the SORT1 gene is located at a locus strongly associated with LDL cholesterol levels in genome-wide association studies.
免疫印迹
WB result of SORT1 Recombinant Rabbit mAb
Primary antibody: SORT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Lane 2: PANC-1 whole cell lysate 20 µg
Lane 3: SK-BR-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 92 kDa
Observed MW: 100 kDaWB result of SORT1 Recombinant Rabbit mAb
Primary antibody: SORT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 92 kDa
Observed MW: 100 kDaWB result of SORT1 Recombinant Rabbit mAb
Primary antibody: SORT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 92 kDa
Observed MW: 100 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SORT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-SORT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SORT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-SORT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Neuro-2a cells. Anti-SORT1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of SORT1 in tumor tissue.
Expression of SORT1 in human tissue.
Expression of SORT1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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