SA2 (isoform-2) Recombinant Rabbit mAb (S-1461-231)

Cohesin subunit SA-2,SCC3 homolog 2,Stromal antigen 2,SA2,STAG2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6081

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

SA2 (isoform-2)
分子别名

Cohesin subunit SA-2; SCC3 homolog 2; Stromal antigen 2; SA2; STAG2
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

Q8N3U4-2
克隆号

S-1461-231
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, K562, 293T, NIH/3T3, mouse spleen
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms
ICC 1:50 Hu, Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms
ICC	1:50	Hu, Ms

背景介绍

SA2 (isoform-2) protein is a variant of the spliceosome-associated protein family, playing a crucial role in pre-mRNA splicing. It is involved in the assembly and function of the spliceosome, a complex molecular machine that removes introns from pre-mRNA transcripts. This protein isoform is essential for accurate and efficient splicing, thereby ensuring proper gene expression. Mutations or dysregulation of SA2 can lead to various cellular defects and may be implicated in certain diseases.

免疫印迹
- WB result of SA2 (isoform-2) Recombinant Rabbit mAb
  Primary antibody: SA2 (isoform-2) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: K562 whole cell lysate 20 µg
  Lane 3: 293T whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 146 kDa
  Observed MW: 146 kDa
- WB result of SA2 (isoform-2) Recombinant Rabbit mAb
  Primary antibody: SA2 (isoform-2) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse spleen lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 146 kDa
  Observed MW: 146 kDa
免疫细胞化学
- ICC shows positive staining in K562 cells. Anti-SA2 (isoform-2) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti-SA2 (isoform-2) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).