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Phospho-Rb (Ser807/811) Recombinant Rabbit mAb (S-2215-52)

Retinoblastoma-associated protein,p105-Rb,p110-RB1,pRb (Rb),pp110,RB1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6076
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-Rb (Ser807/811)

  • 分子别名

    Retinoblastoma-associated protein; p105-Rb; p110-RB1; pRb (Rb); pp110; RB1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P06400

  • 克隆号

    S-2215-52

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    WI-38, C2C12

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms
    IHC-P 1:200 Hu, Ms, Rt
    ICC 1:500 Hu
背景介绍

  • Phospho-Rb (Ser807/811) protein refers to the phosphorylated form of the retinoblastoma protein (Rb) at serine residues 807 and 811. The retinoblastoma protein is a crucial tumor suppressor that plays a pivotal role in regulating cell cycle progression, particularly in controlling the transition from the G1 to S phase. When phosphorylated at Ser807/811, Rb undergoes a conformational change that allows it to release the transcription factor E2F, which in turn promotes cell cycle progression. This phosphorylation is mainly mediated by cyclin-dependent kinases (CDKs) such as CDK4 and CDK6, and it is tightly regulated to ensure proper cell cycle control. Abnormal phosphorylation of Rb at these sites is often associated with various cancers, highlighting its significance in maintaining genomic stability and preventing uncontrolled cell proliferation.

  • 免疫印迹

    • WB result of Phospho-Rb (Ser807/811) Recombinant Rabbit mAb
      Primary antibody: Phospho-Rb (Ser807/811) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated WI-38 whole cell lysate 20 µg
      Lane 2: WI-38 starve for 3 days, then treated with 10 % serum for 2 days whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 106 kDa
      Observed MW: 115 kDa

    • WB result of Phospho-Rb (Ser807/811) Recombinant Rabbit mAb
      Primary antibody: Phospho-Rb (Ser807/811) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated C2C12 whole cell lysate 20 µg
      Lane 2: Lambda phosphatase treated C2C12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 106 kDa
      Observed MW: 115 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti- Phospho-Rb (Ser807/811) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer (Left) and negative staining in human colon cancer treated with phosphatase (Right). Anti- Phospho-Rb (Ser807/811) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse spleen. Anti- Phospho-Rb (Ser807/811) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse stomach. Anti- Phospho-Rb (Ser807/811) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat spleen. Anti- Phospho-Rb (Ser807/811) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC analysis of MCF7 cells treated with Lambda PP for 1 hr (top panel) and MCF7 cells untreated with Lambda PP (below panel). Anti- Phospho-Rb (Ser807/811) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Phospho-Rb (Ser807/811) in tumor tissue.

    • Expression of Phospho-Rb (Ser807/811) in human tissue.

    • Expression of Phospho-Rb (Ser807/811) in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

检测磷酸化蛋白时，推荐有什么封闭体系？

我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高

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