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Rabbit抗原名称
OPA1分子别名
Dynamin-like GTPase OPA1, mitochondrial; Optic atrophy protein 1; KIAA0567免疫原
Synthetic PeptideAccession
O60313克隆号
S-2189-65抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
MCF7, A431, LNCaP, PANC-1, mouse brain, PC-12, rat brain预测反应种属
(反应种属缩写表)Or纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:200 Hu, Ms, Rt
The OPA1 protein, encoded by the OPA1 gene, is a dynamin-related GTPase located in the inner mitochondrial membrane, playing a crucial role in mitochondrial fusion, cristae structure maintenance, and oxidative phosphorylation. It exists in two forms: the long isoform (L-OPA1) that promotes mitochondrial fusion and the short isoform (S-OPA1) generated by proteolytic cleavage, which has recently been shown to maintain mitochondrial energetics and cristae structure under stress conditions. Mutations in the OPA1 gene are associated with optic atrophy type 1, a condition characterized by progressive vision loss due to optic nerve degeneration.
免疫印迹
WB result of OPA1 Recombinant Rabbit mAb
Primary antibody: OPA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: LNCaP whole cell lysate 20 µg
Lane 4: PANC-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 112 kDa
Observed MW: 80-90 kDaWB result of OPA1 Recombinant Rabbit mAb
Primary antibody: OPA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 112 kDa
Observed MW: 80-90 kDaWB result of OPA1 Recombinant Rabbit mAb
Primary antibody: OPA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 112 kDa
Observed MW: 80-90 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human stomach. Anti-OPA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-OPA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-OPA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-OPA1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of OPA1 in tumor tissue.
Expression of OPA1 in human tissue.
Expression of OPA1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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