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宿主来源
Rabbit抗原名称
JAK3分子别名
Tyrosine-protein kinase JAK3; Janus kinase 3 (JAK-3); Leukocyte janus kinase (L-JAK)免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P52333克隆号
S-1979-11抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
TF-1, Jurkat纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
JAK3, which stands for Janus Kinase 3, is a non-receptor tyrosine kinase that plays a crucial role in the immune system. It is predominantly expressed in immune cells such as T cells, B cells, and natural killer cells. JAK3 is involved in the intracellular signaling pathways initiated by cytokine receptors, particularly those of the common gamma chain family, including interleukin-2 (IL-2), IL-4, IL-7, IL-9, IL-15, and IL-21 receptors. Upon receptor activation, JAK3 phosphorylates downstream signaling molecules, leading to the activation of transcription factors like STATs and ultimately regulating cell proliferation, differentiation, and survival. Mutations in the JAK3 gene can result in severe combined immunodeficiency (SCID), highlighting its essential function in immune development and function. Additionally, due to its central role in immune signaling, JAK3 has become a target for therapeutic interventions in autoimmune and inflammatory diseases.
免疫印迹
WB result of JAK3 Recombinant Rabbit mAb
Primary antibody: JAK3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HaCaT whole cell lysate 20 µg
Lane 2: TF-1 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Negative control: HaCaT whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 125 kDa
Observed MW: 115 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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