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宿主来源
Rabbit抗原名称
DAB2分子别名
Disabled homolog 2; Adaptor molecule disabled-2; Differentially expressed in ovarian carcinoma 2 (DOC-2); Differentially-expressed protein 2; DOC2免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P98082克隆号
S-2131-55抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu
Disabled-2 (DAB2) is a multifunctional adaptor protein involved in various cellular processes, including clathrin-mediated endocytosis, signal transduction, and regulation of key signaling pathways such as Wnt, MAPK, and TGF-β. Initially identified as a tumor suppressor in ovarian cancer, DAB2 is downregulated in several cancers, including breast, lung, and colorectal cancer, contributing to tumor progression. It contains functional domains like the phosphotyrosine-binding (PTB) domain and proline-rich SH3 domain, which enable it to interact with multiple proteins and regulate pathways involved in cell differentiation, proliferation, and epithelial-mesenchymal transition. DAB2 also plays a role in platelet activation and aggregation, with different isoforms expressed in human and mouse platelets. Additionally, it is essential for embryonic development and maintaining epithelial cell polarity.
免疫印迹
WB result of DAB2 Recombinant Rabbit mAb
Primary antibody: DAB2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: LNCaP whole cell lysate 10 µg
Lane 2: HeLa whole cell lysate 10 µg
Negative control: LNCaP whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 105 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human placenta. Anti-DAB2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-DAB2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-DAB2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-DAB2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of DAB2 in tumor tissue.
Expression of DAB2 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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