ATM Recombinant Rabbit mAb (S-2178-4)

Serine-protein kinase ATM,Ataxia telangiectasia mutated (A-T mutated)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6072

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

ATM
分子别名

Serine-protein kinase ATM; Ataxia telangiectasia mutated (A-T mutated)
免疫原

Recombinant Protein
细胞定位

Nucleus
Accession

Q13315
克隆号

S-2178-4
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HEK-293, HepG2, HeLa, Raji, MCF7, A549, NIH/3T3, C6
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:50-1:100 Hu, Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:50-1:100	Hu, Ms

背景介绍

The ATM protein, which stands for Ataxia Telangiectasia Mutated protein, is a crucial enzyme involved in the cellular response to DNA damage. It is a serine/threonine kinase that plays a central role in initiating the DNA damage response pathway when double-strand breaks occur in the DNA. Upon activation by DNA damage signals, ATM phosphorylates a wide range of downstream targets, including key proteins like p53, BRCA1, and the MRE11-RAD50-NBS1 complex. These phosphorylation events help to trigger cell cycle arrest, allowing the cell to repair the damaged DNA, and also promote apoptosis if the damage is too severe to be repaired. Mutations in the gene encoding ATM are associated with the rare genetic disorder Ataxia Telangiectasia, which is characterized by neurological defects, immunodeficiency, and an increased risk of cancer, highlighting the essential role of ATM in maintaining genomic stability and preventing tumorigenesis.

免疫印迹
- WB result of ATM Recombinant Rabbit mAb
  Primary antibody: ATM Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Lane 4: Raji whole cell lysate 20 µg
  Lane 5: MCF7 whole cell lysate 20 µg
  Lane 6: A549 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 350 kDa
  Observed MW: 300 kDa
- WB result of ATM Recombinant Rabbit mAb
  Primary antibody: ATM Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 350 kDa
  Observed MW: 300 kDa
- WB result of ATM Recombinant Rabbit mAb
  Primary antibody: ATM Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 350 kDa
  Observed MW: 300 kDa
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti-ATM antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti-ATM antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).