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Rabbit抗原名称
ALOX5分子别名
Polyunsaturated fatty acid 5-lipoxygenase; Arachidonate 5-lipoxygenase (5-LO; 5-lipoxygenase); LOG5免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P09917克隆号
S-1980-27抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
A431, Raji纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200 Hu
ALOX5, also known as 5-lipoxygenase, is a key enzyme involved in the metabolism of arachidonic acid, a polyunsaturated fatty acid. It catalyzes the conversion of arachidonic acid to leukotriene A4, which is then further metabolized into various leukotrienes such as leukotriene B4 and cysteinyl leukotrienes. These leukotrienes play significant roles in inflammatory and allergic responses, including bronchoconstriction, increased vascular permeability, and chemotaxis of immune cells. ALOX5 is primarily expressed in immune cells like neutrophils, macrophages, and mast cells. Its activity is tightly regulated at multiple levels, including transcriptional control, post-translational modifications, and interaction with other proteins. Inhibitors of ALOX5 have been developed as potential therapeutic agents for conditions like asthma, allergic rhinitis, and certain inflammatory diseases, aiming to reduce the production of pro-inflammatory leukotrienes and alleviate associated symptoms.
免疫印迹
WB result of ALOX5 Recombinant Rabbit mAb
Primary antibody: ALOX5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-ALOX5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-ALOX5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-ALOX5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-ALOX5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ALOX5 in tumor tissue.
Expression of ALOX5 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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